Korea created a fluorescent puppy
Korean scientists have created the world's first transgenic dogs. Five beagle puppies carry in their DNA a gene of the red fluorescent protein anemone. An article describing the experimental procedures published in the journal Genesis. Briefly describes the work of the New Scientist.
All puppies were cloned from fibroblasts - connective tissue cells. The nuclei of fibroblasts were removed from the cells and placed in the eggs of other dogs, from which the nuclei were previously removed. Oocytes with “foreign” nuclei were grown in vitro and placed in the uterus to surrogate mothers.
In total, scientists implanted 344 embryos to 20 dogs. Only seven of them succeeded. One of the puppies died in the womb of a surrogate mother, and another died of pneumonia 11 weeks after birth. The remaining five dogs, according to the researchers, are healthy and develop normally. The cloning efficiency was 1.7 percent. By modern standards, it is quite low.
The fluorescent protein gene was inserted into the fibroblast DNA using a specially modified retrovirus. The genetic information of such viruses is not recorded in the DNA molecule, but in the RNA molecule. Retroviruses rewrite their genome into the "language" of DNA and insert it into the genome of the host cell.
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In the case of beagle puppies, scientists could not control where the virus will insert DNA into the host genome. Now they are developing procedures that will allow them to manage this process. Methods for the directional insertion of foreign genetic material have been developed on several mammals, but for dogs they do not yet exist.
Researchers, including Professor Hwang Woo Suk (Hwang Woo-Suk), who tried to falsify the results of experiments with human stem cells and who participated in the creation of the first cloned dog, plan to use transgenic dogs to study human diseases. In their opinion, these animals are more convenient model organisms than, for example, mice. One of the advantages of dogs is a longer life cycle.
Source - lenta.ru
UPD:
One of the transgenic puppies - 10-day Ruppy (Ruppy) - in ultraviolet rays.
Thanks for the photo mihteh
All puppies were cloned from fibroblasts - connective tissue cells. The nuclei of fibroblasts were removed from the cells and placed in the eggs of other dogs, from which the nuclei were previously removed. Oocytes with “foreign” nuclei were grown in vitro and placed in the uterus to surrogate mothers.
In total, scientists implanted 344 embryos to 20 dogs. Only seven of them succeeded. One of the puppies died in the womb of a surrogate mother, and another died of pneumonia 11 weeks after birth. The remaining five dogs, according to the researchers, are healthy and develop normally. The cloning efficiency was 1.7 percent. By modern standards, it is quite low.
The fluorescent protein gene was inserted into the fibroblast DNA using a specially modified retrovirus. The genetic information of such viruses is not recorded in the DNA molecule, but in the RNA molecule. Retroviruses rewrite their genome into the "language" of DNA and insert it into the genome of the host cell.
')
In the case of beagle puppies, scientists could not control where the virus will insert DNA into the host genome. Now they are developing procedures that will allow them to manage this process. Methods for the directional insertion of foreign genetic material have been developed on several mammals, but for dogs they do not yet exist.
Researchers, including Professor Hwang Woo Suk (Hwang Woo-Suk), who tried to falsify the results of experiments with human stem cells and who participated in the creation of the first cloned dog, plan to use transgenic dogs to study human diseases. In their opinion, these animals are more convenient model organisms than, for example, mice. One of the advantages of dogs is a longer life cycle.
Source - lenta.ru
UPD:
One of the transgenic puppies - 10-day Ruppy (Ruppy) - in ultraviolet rays.
Thanks for the photo mihteh
Source: https://habr.com/ru/post/58340/
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