Savings on blood: a new biomaterial delivery system has been developed in the laboratory
Materials scientists of NITU "MISiS" together with colleagues from the chemical faculty of the Moscow State University Lomonosov developed a cheap and high-quality way to deliver blood samples and other biological fluids at any distance for analysis in the laboratory. The method is similar to the technology of obtaining dry blood stains, which has already proven itself in the neonatal screen. Now the professional environment is discussing the possibility of expanding the use of new technology, which, according to the developers, in the near future can supplant traditional methods of sampling and become a global system of screening for infectious diseases (HIV, hepatitis, tuberculosis, etc.).
Currently, medical and veterinary diagnostics in the analysis of blood, as a rule, use special syringes-tubes to obtain liquid samples of blood or serum, which are delivered to the laboratory for analysis in special containers with observance of temperature conditions (often frozen). It is quite expensive and uncomfortable. For example, in the event of an emergency epidemiological situation — an outbreak of anthrax on the Yamal Peninsula in 2016 — the cost of promptly delivering a batch of biomaterial to the Moscow laboratory in the usual way reached several million rubles. In addition, with a possible violation of the temperature or temporary conditions of delivery and storage of blood samples lose their properties, which leads to incorrect results.
One of the solutions to these problems is the increasingly widespread technology for obtaining dry blood stains. However, this method of sampling is not without flaws.
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“The method of obtaining dry blood samples used in screening newborns, when several drops of blood are applied to special cellulose carriers and then dried in air, requires special cards that are not produced in our country, as well as specialized expensive equipment for obtaining biomaterial samples for subsequent analysis. And this is not in every major city , ”says one of the project developers, a senior researcher at the Department of Functional Nano-Systems at NUST“ MISiS ”, Ph.D. Associate Professor Alexander Osipov . “ Moreover, the difficulty of using such carriers for quantitative analysis of the blood of animals and humans is due to the uneven spreading of the carrier on the carrier due to the complex structure of cellulose and the presence of irreversible sorption of blood components, which greatly distorts the results of the analysis .”
Alexander Osipov
The problem was solved by specialists from NUST “MISiS” and Moscow State University, who replaced cellulose with specially prepared porous inorganic material containing metal nanoparticles. Scientists managed to choose the composition and form of the absorber so that the blood is distributed evenly throughout the volume, and when flushing, the analyzed blood components almost completely went into solution.
Modified samples dry approximately 2 times faster, which significantly speeds up the sample preparation process. For transportation to the laboratory, the card is inserted into an envelope and sent by mail without the need for special transport, which leads to significant savings. The cost of the card itself, even with manual small-scale wholesale production, is five times lower than the industrial imported cellulose analogue: 40 rubles versus 180 rubles, when switching to large-scale production, the price will be even lower.
Blood sample card
So far, the creators of the new patented sample preparation technology have decided to concentrate on veterinary diagnostics, because it is in the livestock industry that the need for hundreds of thousands of tests constantly arises, and there are usually no conditions for this on livestock farms located in remote areas.
As rector of NITU “MISiS” Alevtina Chernikova noted, “ within the framework of the Federal Target Program“ Research and Development ”, a group of scientists of NITU“ MISiS ”, under the leadership of Ph.D. population screening. University scientists are already negotiating with leading Russian laboratories, as well as with several large companies in Southeast Asia who are interested in applying the new system both in medical diagnostics and in veterinary medicine . ”
The Federal Center for the Protection of Animals (ARRIAH, Vladimir) became the main partner for the practical testing of the proposed technology. Now several largest poultry and livestock farms of Russia cooperate with scientists, which determine the level of immunity of birds during vaccination, the presence of foot-and-mouth disease viruses, rabies, leukemia and other especially dangerous animal infections. After completion of the tests and certification procedures, it will be possible to start mass production of an experimental series of membrane carriers of a new sample for sampling. According to preliminary estimates, all the necessary documents will be ready by the end of 2017.
____________
Patents:
2014 Device for receiving, storing and transporting dry samples of liquid objects intended for subsequent laboratory analysis
Authors: Osipov A.P., Kondakov S.E., Grigorenko V.G., Smolensky V.I., Prokoptseva OS, Samsonova Zh.V.
RF patent â„–2519030, June 10
2015 KIT FOR RECEPTION, STORAGE AND TRANSPORTATION OF DRY BLOOD SAMPLES FOR FUTURE CONDUCTING LABORATORY ANALYSIS
Authors: Panin A.N., Smolensky V.I., Rudenko T.V., Mitrokhin M.Yu., Samoilov V.E., Kondakov S.E., Grigorenko V.G., Osipov A.P.
RF patent â„–2570750, November 16
Articles:
2016 Porous membrane strip microsampling:
Jeanne Samsonova, Anastasia Chadina, Alexander Osipov, Sergey Kondakov
in Analytical Methods magazine, Royal Society of Chemistry (United Kingdom), vol. 24, no. 8, p. 4835-4843 DOI
2016 A ELISA Bovine Infectious Diseases in Dried Blood Spots
Saushkin N.Yu, Samsonova JV, Osipov AP, Kondakov SE, Efimova MA, Chernov AN
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), Vol. 71, No. 4, p. 253-257 DOI
2016 Comparison of PCR and ELISA Methods for the Detection of Levosis in Dried Blood Spots
Saushkin N.Yu, Samsonova JV, Osipov AP, Kondakov SE, Khammadov NI, Usoltsev KV, Makaev Kh Z., Chernov AN
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), Vol. 71, No. 5-6, p. 319-323 DOI
2016 Modification of Blood [1] Spot Technology by Zinc Oxide Nanoparticles
Samsonova JV, Senatova SI, Muratov DS, Osipov AP, Kondakov SE, Kuznetsov DV
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), Vol. 71, No. 1, p. 60-64 DOI
2016 Diagnosis of infectious diseases of cattle by enzyme immunoassay in dry blood stains using a new sample preparation format
Saushkin N.Yu., Samsonova Z.V., Osipov A.P., Kondakov S.E., Efimova M.A., Chernov A.N.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), Vol. 57, No. 4, p. 263-268
2016 Comparison of PCR and ELISA methods for determining bovine leukemia using dried blood stains
Saushkin N.Yu., Samsonov Z.V., Osipov A.P., Kondakov S.E., Khammadov N.I., Usoltsev K.V., Makaev Kh.Z., Chernov A.N.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), Vol. 57, No. 5, p. 41-47
2015 Membrane materials for storing biological samples intended for comparative nanotoxicological testing
Metelkin A., Kuznetsov D., Kolesnikov E., Chuprunov K., Kondakov S., Osipov A., Samsonova J.
in IOP Conf. Series: Materials Science and Engineering, Vol. 98, No. 012019, p. 1-3 DOI
2015 Modification of membrane materials used in the technology of dry blood stains, zinc oxide nanoparticles
Samsonov ZH.V., Senatova S.I., Muratov DS, Osipov A.P., Kondakov S.E., Kuznetsov D.V.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), Vol. 56, No. 6, p. 418-423
2014 Detection of bovine leukemia virus
Samsonova JV, Chadina AS, Osipov AP, Kondakov SE, Makarova TE, Komarov AB
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), vol. 69, No. 6, p. 282-285 DOI
2014 Detection of antibodies to pathogens of viral etiology in dry blood serum stains of birds
Samsonov ZH.V., Osipov A.P., Kondakov S.E., Rudenko T.V., Smolensky V.I.
in the magazine Poultry, â„– 9, p. 38-42
2014 Determination of bovine leukemia virus by polymerase chain reaction in dry blood stains using a new format membrane system
Samsonov ZH.V., Chadina A.S., Osipov A.P., Kondakov S.E., Makarova T.E., Komarov A.B.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), No. 6, p. 368-372
Currently, medical and veterinary diagnostics in the analysis of blood, as a rule, use special syringes-tubes to obtain liquid samples of blood or serum, which are delivered to the laboratory for analysis in special containers with observance of temperature conditions (often frozen). It is quite expensive and uncomfortable. For example, in the event of an emergency epidemiological situation — an outbreak of anthrax on the Yamal Peninsula in 2016 — the cost of promptly delivering a batch of biomaterial to the Moscow laboratory in the usual way reached several million rubles. In addition, with a possible violation of the temperature or temporary conditions of delivery and storage of blood samples lose their properties, which leads to incorrect results.
One of the solutions to these problems is the increasingly widespread technology for obtaining dry blood stains. However, this method of sampling is not without flaws.
')
“The method of obtaining dry blood samples used in screening newborns, when several drops of blood are applied to special cellulose carriers and then dried in air, requires special cards that are not produced in our country, as well as specialized expensive equipment for obtaining biomaterial samples for subsequent analysis. And this is not in every major city , ”says one of the project developers, a senior researcher at the Department of Functional Nano-Systems at NUST“ MISiS ”, Ph.D. Associate Professor Alexander Osipov . “ Moreover, the difficulty of using such carriers for quantitative analysis of the blood of animals and humans is due to the uneven spreading of the carrier on the carrier due to the complex structure of cellulose and the presence of irreversible sorption of blood components, which greatly distorts the results of the analysis .”
Alexander Osipov
The problem was solved by specialists from NUST “MISiS” and Moscow State University, who replaced cellulose with specially prepared porous inorganic material containing metal nanoparticles. Scientists managed to choose the composition and form of the absorber so that the blood is distributed evenly throughout the volume, and when flushing, the analyzed blood components almost completely went into solution.
Modified samples dry approximately 2 times faster, which significantly speeds up the sample preparation process. For transportation to the laboratory, the card is inserted into an envelope and sent by mail without the need for special transport, which leads to significant savings. The cost of the card itself, even with manual small-scale wholesale production, is five times lower than the industrial imported cellulose analogue: 40 rubles versus 180 rubles, when switching to large-scale production, the price will be even lower.
Blood sample card
So far, the creators of the new patented sample preparation technology have decided to concentrate on veterinary diagnostics, because it is in the livestock industry that the need for hundreds of thousands of tests constantly arises, and there are usually no conditions for this on livestock farms located in remote areas.
As rector of NITU “MISiS” Alevtina Chernikova noted, “ within the framework of the Federal Target Program“ Research and Development ”, a group of scientists of NITU“ MISiS ”, under the leadership of Ph.D. population screening. University scientists are already negotiating with leading Russian laboratories, as well as with several large companies in Southeast Asia who are interested in applying the new system both in medical diagnostics and in veterinary medicine . ”
The Federal Center for the Protection of Animals (ARRIAH, Vladimir) became the main partner for the practical testing of the proposed technology. Now several largest poultry and livestock farms of Russia cooperate with scientists, which determine the level of immunity of birds during vaccination, the presence of foot-and-mouth disease viruses, rabies, leukemia and other especially dangerous animal infections. After completion of the tests and certification procedures, it will be possible to start mass production of an experimental series of membrane carriers of a new sample for sampling. According to preliminary estimates, all the necessary documents will be ready by the end of 2017.
____________
Patents:
2014 Device for receiving, storing and transporting dry samples of liquid objects intended for subsequent laboratory analysis
Authors: Osipov A.P., Kondakov S.E., Grigorenko V.G., Smolensky V.I., Prokoptseva OS, Samsonova Zh.V.
RF patent â„–2519030, June 10
2015 KIT FOR RECEPTION, STORAGE AND TRANSPORTATION OF DRY BLOOD SAMPLES FOR FUTURE CONDUCTING LABORATORY ANALYSIS
Authors: Panin A.N., Smolensky V.I., Rudenko T.V., Mitrokhin M.Yu., Samoilov V.E., Kondakov S.E., Grigorenko V.G., Osipov A.P.
RF patent â„–2570750, November 16
Articles:
2016 Porous membrane strip microsampling:
Jeanne Samsonova, Anastasia Chadina, Alexander Osipov, Sergey Kondakov
in Analytical Methods magazine, Royal Society of Chemistry (United Kingdom), vol. 24, no. 8, p. 4835-4843 DOI
2016 A ELISA Bovine Infectious Diseases in Dried Blood Spots
Saushkin N.Yu, Samsonova JV, Osipov AP, Kondakov SE, Efimova MA, Chernov AN
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), Vol. 71, No. 4, p. 253-257 DOI
2016 Comparison of PCR and ELISA Methods for the Detection of Levosis in Dried Blood Spots
Saushkin N.Yu, Samsonova JV, Osipov AP, Kondakov SE, Khammadov NI, Usoltsev KV, Makaev Kh Z., Chernov AN
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), Vol. 71, No. 5-6, p. 319-323 DOI
2016 Modification of Blood [1] Spot Technology by Zinc Oxide Nanoparticles
Samsonova JV, Senatova SI, Muratov DS, Osipov AP, Kondakov SE, Kuznetsov DV
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), Vol. 71, No. 1, p. 60-64 DOI
2016 Diagnosis of infectious diseases of cattle by enzyme immunoassay in dry blood stains using a new sample preparation format
Saushkin N.Yu., Samsonova Z.V., Osipov A.P., Kondakov S.E., Efimova M.A., Chernov A.N.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), Vol. 57, No. 4, p. 263-268
2016 Comparison of PCR and ELISA methods for determining bovine leukemia using dried blood stains
Saushkin N.Yu., Samsonov Z.V., Osipov A.P., Kondakov S.E., Khammadov N.I., Usoltsev K.V., Makaev Kh.Z., Chernov A.N.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), Vol. 57, No. 5, p. 41-47
2015 Membrane materials for storing biological samples intended for comparative nanotoxicological testing
Metelkin A., Kuznetsov D., Kolesnikov E., Chuprunov K., Kondakov S., Osipov A., Samsonova J.
in IOP Conf. Series: Materials Science and Engineering, Vol. 98, No. 012019, p. 1-3 DOI
2015 Modification of membrane materials used in the technology of dry blood stains, zinc oxide nanoparticles
Samsonov ZH.V., Senatova S.I., Muratov DS, Osipov A.P., Kondakov S.E., Kuznetsov D.V.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), Vol. 56, No. 6, p. 418-423
2014 Detection of bovine leukemia virus
Samsonova JV, Chadina AS, Osipov AP, Kondakov SE, Makarova TE, Komarov AB
in the journal Moscow University Chemistry Bulletin, Allerton Press Inc. (United States), vol. 69, No. 6, p. 282-285 DOI
2014 Detection of antibodies to pathogens of viral etiology in dry blood serum stains of birds
Samsonov ZH.V., Osipov A.P., Kondakov S.E., Rudenko T.V., Smolensky V.I.
in the magazine Poultry, â„– 9, p. 38-42
2014 Determination of bovine leukemia virus by polymerase chain reaction in dry blood stains using a new format membrane system
Samsonov ZH.V., Chadina A.S., Osipov A.P., Kondakov S.E., Makarova T.E., Komarov A.B.
in the journal of the Moscow University Herald. Series 2: Chemistry, publishing house Publishing House Mosk. University (M.), No. 6, p. 368-372
Source: https://habr.com/ru/post/404011/
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